Lehrstuhl für Physikalische Chemie II (Prof. Dr. Dr. Jürgen Popp)
Die Forschung der Arbeitsgruppe konzentriert sich hauptsächlich auf die Entwicklung und Anwendung innovativer Raman-basierter Methoden zur Beantwortung biomedizinischer Fragen. Raman-Spektroskopie und die verschiedenartigen Raman-basierten Technologien wie zum Beispiel die Raman-Mikroskopie, SERS oder CARS sind leistungsfähige Werkzeuge zur Bearbeitung eines breiten Spektrums bioanalytischer und biomedizinischer Probleme wie der schnellen Identifizierung von Pathogenen, der sensitiven Überwachung geringkonzentrierter Moleküle (beispielsweise Drogen oder Metabolite) oder der objektiven klinischen Beurteilung von Zell- und Gewebeproben zur Krebsfrüherkennung.
Raman spectra comparison: cautions and pitfalls of similarity metrics
ErscheinungsjahrErschienen in:Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
S. Mostafapour, A. Mokari, S. Guo, J. Popp, T. Bocklitz
Raman imaging of molecular groups in the wavenumber silent region
ErscheinungsjahrErschienen in:Analytical & bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica
C. Schultz, J. Popp
Raman imaging in the wavenumber silent region emerged around 15 years ago as a powerful tool for visualizing biomolecules and synthetic compounds in complex environments with minimal spectral and biological interference. Since then, the field has advanced from simple proof-of-concept studies using available tags to the rational design of highly efficient Raman labels with sharp silent region signatures, now applied to real biological and biomedical questions. This review traces the evolution from the versatility of label-free Raman to the increasing relevance of labeled strategies, emphasizing how tag design influences functionality, application, and impact. We highlight recent progress in both the synthesis and deployment of tags tailored for specific cellular targets and processes and discuss the emerging need for labeled strategies to meet the demands of sensitivity, multiplexing, and biocompatibility in complex systems. Through this design-to-application perspective, the review provides a comprehensive overview of the current capabilities and significant applications, and identifies key future directions to fully exploit the potential of silent region Raman imaging.
Development of a label-free, functional, molecular and structural imaging system combining optical coherence tomography and Raman spectroscopy for in vivo measurement of rat retina
ErscheinungsjahrErschienen in:Biomedical Optics Express
R. Sentosa, M. Salas, C. Merkle, M. Eibl, W. de Jong, A. Amelink, M. Schmitt, I. Krestnikov, V. Shynkar, M. Kempe, T. Schmoll, B. Baumann, M. Andreana, A. Unterhuber, J. Popp, W. Drexler, R. Leitgeb
In vivo access to molecular information of retinal tissue is considered to play a critical role in enabling early diagnosis of ophthalmic and neurodegenerative diseases. The current gold standard of retina imaging, optical coherence tomography and angiography provides only the retinal morphology and blood perfusion, missing the full spectrum of molecular information. Raman spectroscopy addresses this gap while keeping the investigation non-invasive and label-free. Although previous studies have demonstrated the huge diagnostic potential of combining both modalities for in vivo biological tissue measurement, some have either employed unsafe optical power levels for in vivo retinal measurements or presented results that were negative or contradictory. In this study, we have developed an eye-safe multimodal in vivo label-free imaging system and demonstrate the potential of this device by investigating the retina of a living albino rat. The acquired Raman spectra showed relevant Raman bands in comparison with the previous ex vivo studies. Using this multimodal imaging system for non-invasive retina measurements of transgenic rodents holds the potential to advance the understanding of the pathophysiology of both ophthalmic and neurodegenerative diseases.
Multiplex electrochemical aptasensor for the simultaneous detection of linomycin and neomycin antibiotics
ErscheinungsjahrErschienen in:Talanta: the international journal of pure and applied analytical chemistry
W. Al borhani, A. Rhouati, D. Cialla-May, J. Popp, M. Zourob
Multimodal Optical Imaging Combined with Radiomic Analysis for Fibrotic Cardiac Tissue Investigation
ErscheinungsjahrErschienen in:Analytical Chemistry
A. Krause, G. Giardina, L. Papp, D. Haberl, C. Spielvogel, R. Walton, J. Marchant, N. Pallares-Lupon, K. Kulkarni, X. Li, D. Vasquez, J. Popp, I. Schie, W. Drexler, M. Andreana, A. Unterhuber
