Chair of Physical Chemistry II (Material and Biophotonic)
The research of the working group focuses mainly on the development and application of innovative Raman-based methods to answer biomedical questions. Raman spectroscopy and the various Raman-based technologies such as Raman microscopy, SERS or CARS are powerful tools for addressing a broad range of bioanalytical and biomedical problems such as the rapid identification of pathogens, the sensitive monitoring of low-concentration molecules (e.g. drugs or metabolites) or the objective clinical evaluation of cell and tissue samples for early cancer detection.
Raman spectra comparison: cautions and pitfalls of similarity metrics
Year of publicationPublished in:Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
S. Mostafapour, A. Mokari, S. Guo, J. Popp, T. Bocklitz
Raman imaging of molecular groups in the wavenumber silent region
Year of publicationPublished in:Analytical & bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica
C. Schultz, J. Popp
Raman imaging in the wavenumber silent region emerged around 15 years ago as a powerful tool for visualizing biomolecules and synthetic compounds in complex environments with minimal spectral and biological interference. Since then, the field has advanced from simple proof-of-concept studies using available tags to the rational design of highly efficient Raman labels with sharp silent region signatures, now applied to real biological and biomedical questions. This review traces the evolution from the versatility of label-free Raman to the increasing relevance of labeled strategies, emphasizing how tag design influences functionality, application, and impact. We highlight recent progress in both the synthesis and deployment of tags tailored for specific cellular targets and processes and discuss the emerging need for labeled strategies to meet the demands of sensitivity, multiplexing, and biocompatibility in complex systems. Through this design-to-application perspective, the review provides a comprehensive overview of the current capabilities and significant applications, and identifies key future directions to fully exploit the potential of silent region Raman imaging.
Year of publicationPublished in:Journal of Biophotonics
F. Baldini, K. Dholakia, P. French, O. Guntinas-Lichius, A. Kohler, W. Mäntele, L. Marcu, R. Sroka, S. Umapathy, J. Popp
Characterization of Pro-Drug Metabolism and Drug Permeability Kinetics in a Microphysiological In Vitro Model of the Human Small Intestinal Barrier Incorporating Mucus-Generating Cells Coupled with LC-MS/MS Analysis
Year of publicationPublished in:Advanced Healthcare Materials
S. AlShmmari, R. Fardous, M. ALHamamah, D. Cialla-May, J. Popp, Q. Ramadan, M. Zourob
Multimodal Optical Imaging Combined with Radiomic Analysis for Fibrotic Cardiac Tissue Investigation
Year of publicationPublished in:Analytical Chemistry
A. Krause, G. Giardina, L. Papp, D. Haberl, C. Spielvogel, R. Walton, J. Marchant, N. Pallares-Lupon, K. Kulkarni, X. Li, D. Vasquez, J. Popp, I. Schie, W. Drexler, M. Andreana, A. Unterhuber