Lehrstuhl für Physikalische Chemie II (Prof. Dr. Dr. Jürgen Popp)
Die Forschung der Arbeitsgruppe konzentriert sich hauptsächlich auf die Entwicklung und Anwendung innovativer Raman-basierter Methoden zur Beantwortung biomedizinischer Fragen. Raman-Spektroskopie und die verschiedenartigen Raman-basierten Technologien wie zum Beispiel die Raman-Mikroskopie, SERS oder CARS sind leistungsfähige Werkzeuge zur Bearbeitung eines breiten Spektrums bioanalytischer und biomedizinischer Probleme wie der schnellen Identifizierung von Pathogenen, der sensitiven Überwachung geringkonzentrierter Moleküle (beispielsweise Drogen oder Metabolite) oder der objektiven klinischen Beurteilung von Zell- und Gewebeproben zur Krebsfrüherkennung.
Raman spectra comparison: cautions and pitfalls of similarity metrics
ErscheinungsjahrStatusPrüfung ausstehendErschienen in:Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
S. Mostafapour, A. Mokari, S. Guo, J. Popp, T. Bocklitz
Multiplex electrochemical aptasensor for the simultaneous detection of linomycin and neomycin antibiotics
ErscheinungsjahrErschienen in:Talanta: the international journal of pure and applied analytical chemistry
W. Al borhani, A. Rhouati, D. Cialla-May, J. Popp, M. Zourob
A multiplexing immunosensing platform for the simultaneous detection of snake and scorpion venoms: Towards a better management of antidote administration
ErscheinungsjahrErschienen in:Talanta: the international journal of pure and applied analytical chemistry
A. AlMusharraf, A. Rhouati, D. Cialla-May, J. Popp, M. Zourob
Development of a label-free, functional, molecular and structural imaging system combining optical coherence tomography and Raman spectroscopy for in vivo measurement of rat retina
ErscheinungsjahrErschienen in:Biomedical Optics Express
R. Sentosa, M. Salas, C. Merkle, M. Eibl, W. de Jong, A. Amelink, M. Schmitt, I. Krestnikov, V. Shynkar, M. Kempe, T. Schmoll, B. Baumann, M. Andreana, A. Unterhuber, J. Popp, W. Drexler, R. Leitgeb
In vivo access to molecular information of retinal tissue is considered to play a critical role in enabling early diagnosis of ophthalmic and neurodegenerative diseases. The current gold standard of retina imaging, optical coherence tomography and angiography provides only the retinal morphology and blood perfusion, missing the full spectrum of molecular information. Raman spectroscopy addresses this gap while keeping the investigation non-invasive and label-free. Although previous studies have demonstrated the huge diagnostic potential of combining both modalities for in vivo biological tissue measurement, some have either employed unsafe optical power levels for in vivo retinal measurements or presented results that were negative or contradictory. In this study, we have developed an eye-safe multimodal in vivo label-free imaging system and demonstrate the potential of this device by investigating the retina of a living albino rat. The acquired Raman spectra showed relevant Raman bands in comparison with the previous ex vivo studies. Using this multimodal imaging system for non-invasive retina measurements of transgenic rodents holds the potential to advance the understanding of the pathophysiology of both ophthalmic and neurodegenerative diseases.
Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges
ErscheinungsjahrErschienen in:ACS Applied Materials and Interfaces
L. Lin, R. Alvarez-Puebla, L. Liz-Marzán, M. Trau, J. Wang, L. Fabris, X. Wang, G. Liu, S. Xu, X. Han, L. Yang, A. Shen, S. Yang, Y. Xu, C. Li, J. Huang, S. Liu, J. Huang, I. Srivastava, M. Li, L. Tian, L. Nguyen, X. Bi, D. Cialla-May, P. Matousek, N. Stone, R. Carney, W. Ji, W. Song, Z. Chen, I. Phang, M. Henriksen-Lacey, H. Chen, Z. Wu, H. Guo, H. Ma, G. Ustinov, S. Luo, S. Mosca, B. Gardner, Y. Long, J. Popp, B. Ren, S. Nie, B. Zhao, X. Ling, J. Ye
ErscheinungsjahrErschienen in:Vibrational spectroscopy: an international journal devoted to applications of infrared and Raman spectroscopy
T. Mayerhöfer, S. Pahlow, U. Hübner, J. Popp
Impact of clinical preparation steps and use of sex-specific reference for accurate antibiotic monitoring in body fluids
ErscheinungsjahrErschienen in:Communications Medicine
C. Domes, L. Graul, T. Frosch, J. Popp, S. Hagel, M. Pletz, T. Frosch
Background: Effective antibiotic therapy in critically ill patients requires precise dosing tailored to individual conditions. However, physiological changes in these patients can complicate drug exposure prediction, leading to treatment failure or toxicity. Therapeutic drug monitoring (TDM) is crucial in optimizing antibiotic therapy, with Raman spectroscopy emerging as a promising method due to its speed and sensitivity. Methods: The utility of resonance Raman spectroscopy in analyzing clinical urine samples was investigated, specifically focusing on piperacillin concentrations. Samples subjected to various preparation techniques, including freezing, centrifugation, and filtration, were analyzed using deep UV resonance Raman spectroscopy. Data analysis involved preprocessing and chemometric modeling to assess concentration changes and the influence of sample matrix. Results: Sample preparation steps induce concentration changes in piperacillin, with freezing having the highest impact. Chemometric modeling reveals that freezing, filtration, and centrifugation, especially when combined, reduce drug concentration. Furthermore, the choice of urine reference for quantification impacts results, with sex-specific urine pools showing better accuracy compared to mixed pools. Conclusions: Resonance Raman spectroscopy effectively quantifies piperacillin concentrations in urine. Freezing, centrifugation, and filtration during sample preparation influence drug concentration. Using sex-specific urine pools as references yields more accurate quantification results. These findings underscore the importance of considering sample processing effects and reference selection in TDM studies, offering insights for optimizing antibiotic dosing in critically ill patients. Further validation on a larger scale is warranted to confirm these observations.
