Teilbibliothek Naturwissenschaften: Chemie

Publications

Publications of the Ehricht Group
Teilbibliothek Naturwissenschaften: Chemie
Image: Jan-Peter Kasper (University of Jena)
  • 2024

    1.    Loncaric I, Keinprecht H, Irimaso E, Cabal-Rosel A, Stessl B, Ntakirutimana C, Marek L, Fischer OW, Szostak MP, Oberrauch C, Wittek T, Müller E, Desvars-Larrive A, Feßler AT, Braun SD, Schwarz S, Ehling-Schulz M, Monecke S, Ehricht R, Ruppitsch W, Grunert T, Spergser J. J Appl Microbiol. (2024) Diversity of Staphylococcus aureus isolated from nares of ruminants. 2024 Jan 2;135(1):lxad304. doi: 10.1093/jambio/lxad304. PMID:38159931

    2.    Monecke S, Braun SD, Collatz M, Diezel C, Müller E, Reinicke M, Cabal Rosel A, Feßler AT, Hanke D, Loncaric I, Schwarz S, Cortez de Jäckel S, Ruppitsch W, Gavier-Widén D, Hotzel H, Ehricht R. (2024) Molecular Characterization of Chimeric Staphylococcus aureus Strains from Waterfowl.Microorganisms. 2024 Jan 3;12(1):96. doi: 10.3390/microorganisms12010096. PMID: 38257923 

    3.    Keinprecht H, Irimaso E, Rosel AC, Stessl B, Ntakirutimana C, Marek L, Fischer OW, Szostak MP, Zöchbauer J, Wittek T, Müller E, Desvars-Larrive A, Feßler AT, Braun SD, Schwarz S, Spergser J, Ehling-Schulz M, Monecke S, Ehricht R, Ruppitsch W, Grunert T, Loncaric I. (2024) Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda. J Glob Antimicrob Resist. 2024 Mar;36:326-335. doi: 10.1016/j.jgar.2024.01.017. Epub 2024 Feb 1. 

    4.    Reinicke M, Braun SD, Diezel C, Lemuth O, Engelmann I, Liebe T, Ehricht R. (2024) From Shadows to Spotlight: Enhancing Bacterial DNA Detection in Blood Samples through Cutting-Edge Molecular Pre-Amplification.Antibiotics (Basel). 2024 Feb 6;13(2):161. doi: 10.3390/antibiotics13020161. PMID: 38391548

    5.    Tuchscherr L, Wendler S, Santhanam R, Priese J, Reissig A, Müller E, Ali R, Müller S, Löffler B, Monecke S, Ehricht R, Guntinas-Lichius O. (2024) Reduced Glycolysis and Cytotoxicity in Staphylococcus aureus Isolates from Chronic Rhinosinusitis as Strategies for Host Adaptation. Int J Mol Sci. 2024 Feb 13;25(4):2229. doi: 10.3390/ijms25042229. PMID: 38396905

    6.    Monecke S, Burgold-Voigt S, Braun SD, Diezel C, Liebler-Tenorio EM, Müller E, Nassar R, Reinicke M, Reissig A, Senok A, Ehricht R. (2024) Characterisation of PVL-Positive Staphylococcus argenteus from the United Arab Emirates. Antibiotics (Basel). 2024 Apr 27;13(5):401. doi: 10.3390/antibiotics13050401. PMID: 38786130

    7.    Braun SD, Müller E, Frankenfeld K, Gary D, Monecke S, Ehricht R. (2024) A Proof-of-Concept Protein Microarray-Based Approach for Serotyping of Salmonella enterica Strains. Pathogens. 2024 Apr 25;13(5):355. doi: 10.3390/pathogens13050355. PMID: 38787207

    8.    Timme S, Wendler S, Klassert TE, Saraiva JP, da Rocha UN, Wittchen M, Schramm S, Ehricht R, Monecke S, Edel B, Rödel J, Löffler B, Ramirez MS, Slevogt H, Figge MT, Tuchscherr L. (2024) Competitive inhibition and mutualistic growth in co-infections: deciphering Staphylococcus aureus-Acinetobacter baumannii interaction dynamics. ISME Commun. 2024 Jun 10;4(1):ycae077. doi: 10.1093/ismeco/ycae077. eCollection 2024 Jan. PMID: 38962494

    9.    Monecke S, Boswihi S, Braun SD, Diezel C, Müller E, Reinicke M, Udo E, Ehricht R. (2024) Sequencing a CC239-MRSA-III with a novel composite SCC mec element from Kuwait. Eur J Clin Microbiol Infect Dis. 2024 Sep;43(9):1761-1775. doi: 10.1007/s10096-024-04891-y. Epub 2024 Jul 11. PMID: 38990431

    10.    Reuter C, Hauswald W, Burgold-Voigt S, Hübner U, Ehricht R, Weber K, Popp J. (2024) Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis. Biosensors (Basel). 2024 Aug 17;14(8):398. doi: 10.3390/bios14080398. PMID: 39194627

    11.    Bhat VJ, Blaschke D, Vegesna SV, Burgold-Voigt S, Müller E, Ehricht R, Schmidt H. Sensors (Basel). (2024) Monitoring the Dilution of Buffer Solutions with Different pH Values above and below Physiological pH in Very Small Volumes. 2024 Sep 4;24(17):5751. doi: 10.3390/s24175751. PMID: 39275661 

    12.    Pahlow S, Schmidt S, Pappert T, Thieme L, Makarewicz O, Monecke S, Ehricht R, Weber K, Popp J. (2024) Evaluating the potential of vancomycin-modified magnetic beads as a tool for sample preparation in diagnostic assays. Anal Methods. 2024 Oct 31;16(42):7148-7160. doi: 10.1039/d4ay01557f. PMID: 39295576

    13.    Loncaric I, Szostak MP, Cabal-Rosel A, Grünzweil OM, Riegelnegg A, Misic D, Müller E, Feßler AT, Braun SD, Schwarz S, Monecke S, Ehricht R, Ruppitsch W, Spergser J, Lewis A, Bloom PH, Saggese MD. (2024) Molecular characterization, virulence and antimicrobial and biocidal susceptibility of selected bacteria isolated from the cloaca of nestling ospreys (Pandion haliaetus) from Mono Lake, California, USA. PLoS One. 2024 Sep 27;19(9):e0311306. doi: 10.1371/journal.pone.0311306. eCollection 2024. PMID: 39331631 

    14.    Osadare IE, Monecke S, Abdilahi A, Müller E, Collatz M, Braun S, Reissig A, Schneider-Brachert W, Kieninger B, Eichner A, Rath A, Fritsch J, Gary D, Frankenfeld K, Wellhöfer T, Ehricht R. (2024) Fast and Economic Microarray-Based Detection of Species-, Resistance-, and Virulence-Associated Genes in Clinical Strains of Vancomycin-Resistant Enterococci (VRE). Sensors (Basel). 2024 Oct 8;24(19):6476. doi: 10.3390/s24196476. PMID: 39409516

    15.    Lohde M, Wagner GE, Dabernig-Heinz J, Viehweger A, Braun SD, Monecke S, Diezel C, Stein C, Marquet M, Ehricht R, Pletz MW, Brandt C. (2024) Accurate bacterial outbreak tracing with Oxford Nanopore sequencing and reduction of methylation-induced errors. Genome Res. 2024 Oct 23. doi: 10.1101/gr.278848.123. Online ahead of print. PMID: 39443152

    16.    Collatz, M., Reinicke, M., Diezel, C., Braun, S. D., Monecke, S., Reissig, A., & Ehricht, R. (2024). ConsensusPrime—A Bioinformatic Pipeline for Efficient Consensus Primer Design—Detection of Various Resistance and Virulence Factors in MRSA—A Case Study. BioMedInformatics, 4(2), 1249-1261.

    17.    Julián Henao-Restrepo, Anna Schneider, Sebastian Krautwurst, Celia Diezel, Milena Zarkovic, Rodolfo R. Favaro, Manja Marz, Diana M. Morales-Prieto, Udo R. Markert (2024) Analysis of Non-Coding RNAs in Placental Tissue and Extracellular Vesicles: Implications for Trophoblast Function. ;Placenta; Volume 154; 2024, e57-e58; ISSN 0143-4004; doi: /10.1016/j.placenta.2024.07.224.

    18.    Irimaso E, Keinprecht H, Szostak MP, Rosel AC, Stessl B, Desvars-Larrive A, Ntakirutimana C, Fischer OW, Wittek T, Müller E, Feßler AT, Braun SD, Schwarz S, Monecke S, Ehricht R, Spergser J, Ruppitsch W, Loncaric I. (2024) Survey in ruminants from Rwanda revealed high diversity and prevalence of extended-spectrum cephalosporin-resistant Enterobacterales. BMC Vet Res. 2024 Nov 19;20(1):523. doi: 10.1186/s12917-024-04359-3

    19.    Braun, S.D.; Rezk, S.; Brandt, C.; Reinicke, M.; Diezel, C.; Müller, E.; Frankenfeld, K.; Krähmer, D.; Monecke, S.; Ehricht, R. (2024) Tracking Multidrug Resistance in Gram-Negative Bacteria in Alexandria, Egypt (2020–2023): An Integrated Analysis of Patient Data and Diagnostic Tools. Antibiotics 2024, 13, 1185. doi: 10.3390/antibiotics13121185

  • 2023
    1. Szumlanski T, Neumann B, Bertram R, Simbeck A, Ziegler R, Monecke S, Ehricht R, Schneider-Brachert W, Steinmann J. (2023) Characterization of PVL-Positive MRSA Isolates in Northern Bavaria, Germany over an Eight-Year Period. Microorganisms. 2022 Dec 24;11(1):54. doi: 10.3390/microorganisms11010054.
    2. Monecke S, Bedewy AK, Müller E, Braun SD, Diezel C, Elsheredy A, Kader O, Reinicke M, Ghazal A, Rezk S, Ehricht R. (2023) Characterisation of Methicillin- Resistant Staphylococcus aureus from Alexandria, Egypt. Antibiotics (Basel). 2023 Jan 1;12(1):78. doi: 10.3390/antibiotics12010078.
    3. Wagner GE, Berner A, Lipp M, Kohler C, Assig K, Lichtenegger S, Saqib M, Müller E, Trinh TT, Gad AM, Söffing HH, Ehricht R, Laroucau K, Steinmetz I. (2023) Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics. J Clin Microbiol. 2023 Jan 26;61(1):e0123422. doi: 10.1128/jcm.01234-22.
    4. Aloba BK, Kinnevey PM, Monecke S, Brennan GI, O'Connell B, Blomfeldt A, McManus BA, Schneider-Brachert W, Tkadlec J, Ehricht R, Senok A, Bartels MD, Coleman DC. (2023) An emerging Panton-Valentine leukocidin-positive CC5-meticillin- resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing. J Hosp Infect. 2023 Feb;132:8-19. doi: 10.1016/j.jhin.2022.11.015
    5. Wagner GE, Dabernig-Heinz J, Lipp M, Cabal A, Simantzik J, Kohl M, Scheiber M, Lichtenegger S, Ehricht R, Leitner E, Ruppitsch W, Steinmetz I. (2023) Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance. J Clin Microbiol. 2023 Apr 20;61(4):e0163122. doi: 10.1128/jcm.01631-22.
    6. Burgold-Voigt S, Monecke S, Busch A, Bocklisch H, Braun SD, Diezel C, Hotzel H, Liebler-Tenorio EM, Müller E, Reinicke M, Reissig A, Ruppelt-Lorz A, Ehricht R. (2023) Characterisation of a <i>Staphylococcus aureus</i> Isolate Carrying Phage-Borne Enterotoxin E from a European Badger (Meles meles). Pathogens. 2023 May 12;12(5):704. doi: 10.3390/pathogens12050704.
    7. Pahlow S, Richard-Lacroix M, Hornung F, Köse-Vogel N, Mayerhöfer TG, Hniopek J, Ryabchykov O, Bocklitz T, Weber K, Ehricht R, Löffler B, Deinhardt-Emmer S, Popp J. (2023) Simple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy. Biosensors (Basel). 2023 May 30;13(6):594. doi: 10.3390/bios13060594.
    8. Monecke S, Akpaka PE, Smith MR, Unakal CG, Thoms Rodriguez CA, Ashraph K, Müller E, Braun SD, Diezel C, Reinicke M, Ehricht R. (2023) Clonal Complexes Distribution of Staphylococcus aureus Isolates from Clinical Samples from the Caribbean Islands. Antibiotics (Basel). 2023 Jun 14;12(6):1050. doi: 10.3390/antibiotics12061050.
    9. Bhat VJ, Blaschke D, Müller E, Ehricht R, Schmidt H. (2023) A Novel Approach to Monitor the Concentration of Phosphate Buffers in the Range of 1 M to 0.1 M Using a Silicon-Based Impedance Sensor. Biosensors (Basel). 2023 Aug 24;13(9):841. doi: 10.3390/bios13090841.
    10. Braun SD, Asif M, Hassan L, Ul Haq I, Abbasi SA, Jamil B, Monecke S, Ehricht R, Mueller E, Syed MA.(2023) Analysis of carbapenem-resistant strains isolated in a tertiary care hospital in Rawalpindi, Pakistan, during the years 2016 and 2020. J Hosp Infect. 2023 Nov;141:187-189. doi: 10.1016/j.jhin.2023.06.007.
    11. Moawad AA, El-Adawy H, Linde J, Jost I, Gärtner T, Hruschka K, Donat K, Neubauer H, Monecke S, Tomaso H (2023) Whole genome sequence-based analysis of Staphylococcus aureus isolated from bovine mastitis in Thuringia, Germany Front. Microbiol., 25 August 2023 Sec. Antimicrobials, Resistance and Chemotherapy, doi: 10.3389/fmicb.2023.1216850
    12. Osadare IE, Xiong L, Rubio I, Neugebauer U, Press AT, Ramoji A, Popp J. (2023) Raman Spectroscopy Profiling of Splenic T-Cells in Sepsis and Endotoxemia in Mice Int J Mol Sci 2023 Jul 27;24(15):12027. doi: 10.3390/ijms241512027. PMID: 37569403 PMCID: PMC10419286 DOI: 10.3390/ijms241512027
    13. Triebel S, Sachse K, Weber M, Heller M, Diezel C, Hölzer M, Schnee C, Marz M. (2023) De novo genome assembly resolving repetitive structures enables genomic analysis of 35 European Mycoplasmopsis bovis strains. BMC Genomics  2023 Sep 16;24(1):548. doi: 10.1186/s12864-023-09618-5.
    14. Loncaric I, Keinprecht H, Irimaso E, Cabal-Rosel A, Stessl B, Ntakirutimana C, Marek L, Fischer O, Szostak M, Oberrauch C, Wittek T, Müller E, Desvars A, Feßler A, Braun S, Schwarz, S, Ehling-Schultz M, Monecke S, Ehricht R, Ruppitsch W, Grunert T, Spergser J. (2023) Diversity of Staphylococcus aureus isolated from nares of ruminants Journal of Applied Microbiology - Decision on Manuscript ID JAMICRO-2023-0994.R1
  • 2022
    1. Monecke S, Feßler AT, Burgold-Voigt S, Krüger H, Mühldorfer K, Wibbelt G, Liebler-Tenorio EM, Reinicke M, Braun SD, Hanke D, Diezel C, Müller E, Loncaric I, Schwarz S, Ehricht R. (2021) Staphylococcus aureus isolates from Eurasian Beavers (Castor fiber) carry a novel phage-borne bicomponent leukocidin related to the Panton-Valentine leukocidin. Sci Rep. 2021 Dec 22;11(1):24394. doi: 10.1038/s41598-021-03823-6.
    2. Tsilipounidaki K, Athanasakopoulou Z, Müller E, Burgold-Voigt S, Florou Z, Braun SD, Monecke S, Gatselis NK, Zachou K, Stefos A, Tsagalas I, Sofia M, Spyrou V, Billinis C, Dalekos GN, Ehricht R, Petinaki E. (2022) Plethora of Resistance Genes in Carbapenem-Resistant Gram-Negative Bacteria in Greece: No End to a Continuous Genetic Evolution. Microorganisms. 2022 Jan 13;10(1):159. doi: 10.3390/microorganisms10010159.
    3. El-Ashker M, Monecke S, Gwida M, Saad T, El-Gohary A, Mohamed A, Reißig A, Frankenfeld K, Gary D, Müller E, Ehricht R. (2022) Molecular characterisation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus clones isolated from healthy dairy animals and their caretakers in Egypt. Vet Microbiol. 2022 Apr;267:109374. doi: 10.1016/j.vetmic.2022.109374. Epub 2022 Feb
    4. Luzzago C, Lauzi S, Ehricht R, Monecke S, Corlatti L, Pedrotti L, Piccinini R. Survey of Staphylococcus aureus carriage by free-living red deer (Cervus elaphus): Evidence of human and domestic animal lineages. (2022) Transbound Emerg Dis. 2022 Sep;69(5):e1659-e1669. doi: 10.1111/tbed.14500. Epub 2022 Mar 22.
    5. Marquet M, Zöllkau J, Pastuschek J, Viehweger A, Schleußner E, Makarewicz O, Pletz MW, Ehricht R, Brandt C. Evaluation of microbiome enrichment and host DNA depletion in human vaginal samples using Oxford Nanopore's adaptive sequencing. (2022) Sci Rep. 2022 Mar 7;12(1):4000. doi: 10.1038/s41598-022-08003-8.
    6. Finsterwalder SK, Loncaric I, Cabal A, Szostak MP, Barf LM, Marz M, Allerberger F, Burgener IA, Tichy A, Feßler AT, Schwarz S, Monecke S, Ehricht R, Ruppitsch W, Spergser J, Künzel F. (2022) Dogs as carriers of virulent and resistant genotypes of Clostridioides difficile. Zoonoses Public Health. 2022 Sep;69(6):673-681. doi: 10.1111/zph.12956. Epub 2022 May 12. PMID: 35546073;
    7. Burgold-Voigt S, Müller E, Zopf D, Monecke S, Braun SD, Frankenfeld K, Kiehntopf M, Weis S, Schumacher T, Pletz MW, Ehricht R; CoNAN Study Group. Development of a new antigen-based microarray platform for screening and detection of human IgG antibodies against SARS-CoV-2. (2022) Sci Rep. 2022 May 16;12(1):8067. doi: 10.1038/s41598-022-10823-7.
    8. Monecke S, Schaumburg F, Shittu AO, Schwarz S, Mühldorfer K, Brandt C, Braun SD, Collatz M, Diezel C, Gawlik D, Hanke D, Hotzel H, Müller E, Reinicke M, Feßler AT, Ehricht R. (2022) Description of Staphylococcal Strains from Straw-Coloured Fruit Bat (Eidolon helvum) and Diamond Firetail (Stagonopleura guttata) and a Review of their Phylogenetic Relationships to Other Staphylococci. Front Cell Infect Microbiol. 2022 May 11;12:878137. doi: 10.3389/fcimb.2022.878137.
    9. Tsekouras N, Athanasakopoulou Z, Diezel C, Kostoulas P, Braun SD, Sofia M, Monecke S, Ehricht R, Chatzopoulos DC, Gary D, Krähmer D, Spyrou V, Christodoulopoulos G, Billinis C, Papatsiros VG. (2022) Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece. (2022) Animals (Basel). 2022 Jun 16;12(12):1560. doi: 10.3390/ani12121560.
    10. Athanasakopoulou Z, Diezel C, Braun SD, Sofia M, Giannakopoulos A, Monecke S, Gary D, Krähmer D, Chatzopoulos DC, Touloudi A, Birtsas P, Palli M, Georgakopoulos G, Spyrou V, Petinaki E, Ehricht R, Billinis C. (2022) Occurrence and Characteristics of ESBL- and Carbapenemase- Producing Escherichia coli from Wild and Feral Birds in Greece. Microorganisms. 2022 Jun 14;10(6):1217. doi: 10.3390/microorganisms10061217.
    11. Pistiki A, Monecke S, Shen H, Ryabchykov O, Bocklitz TW, Rösch P, Ehricht R, Popp J. (2022) Comparison of Different Label-Free Raman Spectroscopy Approaches for the Discrimination of Clinical MRSA and MSSA Isolates. (2022) Microbiol Spectr. 2022 Oct 26;10(5):e0076322. doi: 10.1128/spectrum.00763-22. Epub 2022 Aug 25.
    12. Moawad AA, Hotzel H, Hafez HM, Ramadan H, Tomaso H, Braun SD, Ehricht R, Diezel C, Gary D, Engelmann I, Zakaria IM, Reda RM, Eid S, Shahien MA, Neubauer H, Monecke S. (2022) Occurrence, Phenotypic and Molecular Characteristics of Extended- Spectrum Beta-Lactamase-Producing Escherichia coli in Healthy Turkeys in Northern Egypt. Antibiotics (Basel). 2022 Aug 8;11(8):1075. doi: 10.3390/antibiotics11081075.
    13. Monecke S, Roberts MC, Braun SD, Diezel C, Müller E, Reinicke M, Linde J, Joshi PR, Paudel S, Acharya M, Chalise MK, Feßler AT, Hotzel H, Khanal L, Koju NP, Schwarz S, Kyes RC, Ehricht R. (2022) Sequence Analysis of Novel Staphylococcus aureus Lineages from Wild and Captive Macaques. (2022) Int J Mol Sci. 2022 Sep 23;23(19):11225. doi: 10.3390/ijms231911225.
    14. Steinig E, Aglua I, Duchene S, Meehan MT, Yoannes M, Firth C, Jaworski J, Drekore J, Urakoko B, Poka H, Wurr C, Ebos E, Nangen D, Müller E, Mulvey P, Jackson C, Blomfeldt A, Aamot HV, Laman M, Manning L, Earls M, Coleman DC, Greenhill A, Ford R, Stegger M, Syed MA, Jamil B, Monecke S, Ehricht R, Smith S, Pomat W, Horwood P, Tong SYC, McBryde E. (2022) Phylodynamic signatures in the emergence of community-associated MRSA. Proc Natl Acad Sci U S A. 2022 Nov 8;119(45):e2204993119. doi: 10.1073/pnas.2204993119. Epub 2022 Nov 2.
    15. Marquet M, Hölzer M, Pletz MW, Viehweger A, Makarewicz O, Ehricht R, Brandt C. (2022) What the Phage: a scalable workflow for the identification and analysis of phage sequences. Gigascience. 2022 Nov 18;11:giac110. doi: 10.1093/gigascience/giac110.
    16. Aloba BK, Kinnevey PM, Monecke S, Brennan GI, O'Connell B, Blomfeldt A, McManus BA, Schneider-Brachert W, Tkadlec J, Ehricht R, Senok A, Bartels MD, Coleman DC. (2022) An emerging Panton-Valentine leukocidin (PVL)-positive CC5-meticillin-resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing. J Hosp Infect. 2022 Dec 5:S0195-6701(22)00369-3. doi: 10.1016/j.jhin.2022.11.015. Epub ahead of print.
    17. Collatz M, Braun SD, Monecke S and Ehricht R (2022); ConsensusPrime—A Bioinformatic Pipeline for Ideal Consensus Primer Design.; BioMedInformatics 2022, 2(4), 637-642; doi: 10.3390/biomedinformatics2040041
    18. Akarsu H, Liljander A, Younan M, Brodard I, Overesch G, Glücks I, Labroussaa F, Kuhnert P, Perreten V, Monecke S, Drexler JF, Corman VM, Falquet L, Jores J. (2022) Genomic Characterization and Antimicrobial Susceptibility of Dromedary- Associated Staphylococcaceae from the Horn of Africa. Appl Environ Microbiol. 2022 Nov 8;88(21):e0114622. doi: 10.1128/aem.01146-22. Epub 2022 Oct 13.
    19. Engler C, Renna MS, Beccaria C, Silvestrini P, Pirola SI, Pereyra EAL, Baravalle C, Camussone CM, Monecke S, Calvinho LF, Dallard BE. Differential immune response to two Staphylococcus aureus strains with distinct adaptation genotypes after experimental intramammary infection of dairy cows. Microb Pathog. 2022 Nov;172:105789. doi: 10.1016/j.micpath.2022.105789. Epub 2022 Sep 20.
  • 2021
    • Molecular characterisation of extended-spectrum ß-lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria. Fashae K, Engelmann I, Monecke S, Braun SD, Ehricht R. BMC Vet Res. 2021 Jan 18;17(1):33. doi: 10.1186/s12917-020-02734-4. PMID: 33461554
    • Multiple distinct outbreaks of Panton-Valentine leucocidin-positive community-associated meticillin-resistant Staphylococcus aureus in Ireland investigated by whole-genome sequencing. McManus BA, Aloba BK, Earls MR, Brennan GI, O'Connell B, Monecke S, Ehricht R, Shore AC, Coleman DC. J Hosp Infect. 2021 Feb;108:72-80. doi: 10.1016/j.jhin.2020.11.021. Epub 2020 Nov 28. PMID: 33259881
    • The Pheno- and Genotypic Characterization of Porcine Escherichia coli Isolates. Bernreiter-Hofer T, Schwarz L, Müller E, Cabal-Rosel A, Korus M, Misic D, Frankenfeld K, Abraham K, Grünzweil O, Weiss A, Feßler AT, Allerberger F, Schwarz S, Szostak MP, Ruppitsch W, Ladinig A, Spergser J, Braun SD, Monecke S, Ehricht R, Loncaric I. Microorganisms. 2021 Aug 6;9(8):1676. doi: 10.3390/microorganisms9081676. PMID: 34442755
    • Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals. Grünzweil OM, Palmer L, Cabal A, Szostak MP, Ruppitsch W, Kornschober C, Korus M, Misic D, Bernreiter-Hofer T, Korath ADJ, Feßler AT, Allerberger F, Schwarz S, Spergser J, Müller E, Braun SD, Monecke S, Ehricht R, Walzer C, Smodlaka H, Loncaric I. Int J Mol Sci. 2021 May 31;22(11):5905. doi: 10.3390/ijms22115905. PMID: 34072783
    • MRSA improvement within a highly endemic hospital in Malta: infection control measures or clonal change? Borg MA, Monecke S, Haider J, Müller E, Reissig A, Ehricht R. J Hosp Infect. 2021 Apr; 110:201-202. doi: 10.1016/j.jhin.2021.02.003. Epub 2021 Feb 9. PMID: 33577835 No abstract available.
    • The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia. Mišić D, Kiskaroly F, Szostak MP, Cabal A, Ruppitsch W, Bernreiter-Hofer T, Milovanovic V, Feßler AT, Allerberger F, Spergser J, Müller E, Schwarz S, Braun SD, Monecke S, Ehricht R, Korus M, Benković D, Korzeniowska M, Loncaric I. Antibiotics (Basel). 2021 Sep 3;10(9):1063. doi: 10.3390/antibiotics10091063. PMID: 34572647
    • Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece. Athanasakopoulou Z, Reinicke M, Diezel C, Sofia M, Chatzopoulos DC, Braun SD, Reissig A, Spyrou V, Monecke S, Ehricht R, Tsilipounidaki K, Giannakopoulos A, Petinaki E, Billinis C. Antibiotics (Basel). 2021 Apr 4;10(4):389. doi: 10.3390/antibiotics10040389. PMID: 33916633 Free PMC article.
    • Molecular Characterization of Staphylococcus aureus Isolated from Human and Food Samples in Northern Algeria. Achek R, El-Adawy H, Hotzel H, Hendam A, Tomaso H, Ehricht R, Neubauer H, Nabi I, Hamdi TM, Monecke S. Pathogens. 2021 Oct 3;10(10):1276. doi: 10.3390/pathogens10101276. PMID: 34684225
    • Lateral Flow Immunoassay for the Detection of Panton-Valentine Leukocidin in Staphylococcus aureus From Skin and Soft Tissue Infections in the United Arab Emirates. Senok A, Monecke S, Nassar R, Celiloglu H, Thyagarajan S, Müller E, Ehricht R. Front Cell Infect Microbiol. 2021 Oct 18;11:754523. doi: 10.3389/fcimb.2021.754523. eCollection 2021. PMID: 34733796
    • Assessment of Phenotype Relevant Amino Acid Residues in TEM-β-Lactamases by Mathematical Modelling and Experimental Approval.Madzgalla S, Duering H, Hey JC, Neubauer S, Feller KH, Ehricht R, Pletz MW, Makarewicz O. Microorganisms. 2021 Aug 13;9(8):1726. doi: 10.3390/microorganisms9081726. PMID: 34442804
    • Characterisation of S. aureus/MRSA CC1153 and review of mobile genetic elements carrying the fusidic acid resistance gene fusC. Monecke S, Müller E, Braun SD, Armengol-Porta M, Bes M, Boswihi S, El-Ashker M, Engelmann I, Gawlik D, Gwida M, Hotzel H, Nassar R, Reissig A, Ruppelt-Lorz A, Senok A, Somily AM, Udo EE, Ehricht R. Sci Rep. 2021 Apr 14;11(1):8128. doi: 10.1038/s41598-021-86273-4. PMID: 33854075
    • The Dissemination and Molecular Characterization of Clonal Complex 361 (CC361) Methicillin-Resistant Staphylococcus aureus (MRSA) in Kuwait Hospitals. Sarkhoo E, Udo EE, Boswihi SS, Monecke S, Mueller E, Ehricht R. Front Microbiol. 2021 May 6;12:658772. doi: 10.3389/fmicb.2021.658772. eCollection 2021. PMID: 34025612
    • Exploring the evolution and epidemiology of European CC1-MRSA-IV: tracking a multidrug-resistant community-associated meticillin-resistant Staphylococcus aureus clone. Earls MR, Steinig EJ, Monecke S, Samaniego Castruita JA, Simbeck A, Schneider-Brachert W, Vremerǎ T, Dorneanu OS, Loncaric I, Bes M, Lacoma A, Prat Aymerich C, Wernery U, Armengol-Porta M, Blomfeldt A, Duchene S, Bartels MD, Ehricht R, Coleman DC. Microb Genom. 2021 Jul;7(7):000601. doi: 10.1099/mgen.0.000601. PMID: 34223815
    • ε2-Phages Are Naturally Bred and Have a Vastly Improved Host Range in Staphylococcus aureus over Wild Type Phages. Sáez Moreno D, Visram Z, Mutti M, Restrepo-Córdoba M, Hartmann S, Kremers AI, Tišáková L, Schertler S, Wittmann J, Kalali B, Monecke S, Ehricht R, Resch G, Corsini L. Pharmaceuticals (Basel). 2021 Apr 2;14(4):325. doi: 10.3390/ph14040325. PMID: 33918287
    • Diversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids. Schauer B, Szostak MP, Ehricht R, Monecke S, Feßler AT, Schwarz S, Spergser J, Krametter-Frötscher R, Loncaric I. Vet Microbiol. 2021 Mar;254:109005. doi: 10.1016/j.vetmic.2021.109005. Epub 2021 Feb 4. PMID: 33582485
    • Long-Term Sinonasal Carriage of Staphylococcus aureus and Anti-Staphylococcal Humoral Immune Response in Patients with Chronic Rhinosinusitis. Thunberg U, Hugosson S, Ehricht R, Monecke S, Müller E, Cao Y, Stegger M, Söderquist B. Microorganisms. 2021 Jan 27;9(2):256. doi: 10.3390/microorganisms9020256. PMID: 33513900
    • Hepatectomy-Induced Alterations in Hepatic Perfusion and Function - Toward Multi-Scale Computational Modeling for a Better Prediction of Post-hepatectomy Liver Function. Christ B, Collatz M, Dahmen U, Herrmann K-H, Höpfl S, König M, Lambers L, Marz M, Meyer D, Radde N, Reichenbach JR, Ricken T and Tautenhahn H-M (2021) Front. Physiol. 12:733868. doi: 10.3389/fphys.2021.733868
    • Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages. Burgold-Voigt S, Monecke S, Simbeck A, Holzmann T, Kieninger B, Liebler-Tenorio EM, Braun SD, Collatz M, Diezel C, Müller E, Schneider-Brachert W and Ehricht R (2021) Front. Genet. 12:723958. doi: 10.3389/fgene.2021.723958
    • Characterization of Enterococci- and ESBL-Producing Escherichia coli Isolated from Milk of Bovides with Mastitis in Egypt. Ahmed W, Neubauer H, Tomaso H, El Hofy FI, Monecke S, Abd El-Tawab AA, Hotzel H. Pathogens. 2021 Jan 21;10(2):97. doi: 10.3390/pathogens10020097. PMID: 33494211
  • 2020
    1. Antók FI, Mayrhofer R, Marbach H, Masengesho JC, Keinprecht H, Nyirimbuga V, Fischer O, Lepuschitz S, Ruppitsch W, Ehling-Schulz M, Feßler AT, Schwarz S, Monecke S, Ehricht R, Grunert T, Spergser J, Loncaric I. (2020) Characterization of Antibiotic and Biocide Resistance Genes and Virulence Factors of Staphylococcus Species Associated with Bovine Mastitis in Rwanda. Antibiotics (Basel). 2019 Dec 18;9(1):1. doi: 10.3390/antibiotics9010001
    2. Gwida M, Awad A, El-Ashker M, Hotzel H, Monecke S, Ehricht R, Müller E, Reißig A, Barth SA, Berens C, Braun SD. (2020) Microarray-based detection of resistance and virulence factors in commensal Escherichia coli from livestock and farmers in Egypt. Vet Microbiol. 2020 Jan;240:108539. doi: 10.1016/j.vetmic.2019.108539
    3. El-Ashker M, Gwida M, Monecke S, El-Gohary F, Ehricht R, Elsayed M, Akinduti P, El-Fateh M, Maurischat S. (2020) Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt. Vet Microbiol. 2020 Jan;240:108535. doi: 10.1016/j.vetmic.2019.108535
    4. Senok A, Nassar R, Kaklamanos EG, Belhoul K, Abu Fanas S, Nassar M, Azar AJ, Müller E, Reissig A, Gawlik D, Monecke S, Ehricht R. (2020) Molecular Characterization of Staphylococcus aureus Isolates Associated with Nasal Colonization and Environmental Contamination in Academic Dental Clinics. Microb Drug Resist. 2020 Jun;26(6):661-669. doi: 10.1089/mdr.2019.0318
    5. Achek R, Hotzel H, Nabi I, Kechida S, Mami D, Didouh N, Tomaso H, Neubauer H, Ehricht R, Monecke S, El-Adawy H. (2020)Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria. Pathogens. 2020 Feb 24;9(2):153. doi: 10.3390/pathogens9020153
    6. Loreck K, Mitrenga S, Heinze R, Ehricht R, Engemann C, Lueken C, Ploetz M, Greiner M, Meemken D. (2020) Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs. BMC Vet Res. 2020 Apr 6;16(1):106. doi: 10.1186/s12917-020-02308-4
    7. Rödel J, Edel B, Braun SD, Ehricht R, Simon S, Fruth A, Löffler B. (2020) Simple differentiation of Salmonella Typhi, Paratyphi and Choleraesuis from Salmonella species using the eazyplex TyphiTyper LAMP assay. J Med Microbiol. 2020 Jun;69(6):817-823. doi: 10.1099/jmm.0.001201
    8. Monecke S, König E, Earls MR, Leitner E, Müller E, Wagner GE, Poitz DM, Jatzwauk L, Vremerǎ T, Dorneanu OS, Simbeck A, Ambrosch A, Zollner-Schwetz I, Krause R, Ruppitsch W, Schneider-Brachert W, Coleman DC, Steinmetz I, Ehricht R. (2020) An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020. Euro Surveill. 2020;25(25):2000929. doi: 10.2807/1560-7917.ES.2020.25.25.2000929
    9. Wagner GE, Föderl-Höbenreich E, Assig K, Lipp M, Berner A, Kohler C, Lichtenegger S, Stiehler J, Karoonboonyanan W, Thanapattarapairoj N, Promkong C, Koosakulnirand S, Chaichana P, Ehricht R, Gad AM, Söffing HH, Dunachie SJ, Chantratita N, Steinmetz I. (2020) Melioidosis DS rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection. PLoS Negl Trop Dis. 2020 Jul 13;14(7):e0008452. doi: 10.1371/journal.pntd.0008452
    10. El-Ashker M, Gwida M, Monecke S, Ehricht R, Elsayed M, El-Gohary F, Reißig A, Müller E, Paul A, Igbinosa EO, Beshiru A, Maurischat S. (2020) Microarray-based detection of resistance genes in coagulase-negative staphylococci isolated from cattle and buffalo with mastitis in Egypt. Trop Anim Health Prod. 2020 Nov;52(6):3855-3862. doi: 10.1007/s11250-020-02424-1
    11. Gawlik D, Ruppelt-Lorz A, Müller E, Reißig A, Hotzel H, Braun SD, Söderquist B, Ziegler-Cordts A, Stein C, Pletz MW, Ehricht R, Monecke S. (2020) Molecular investigations on a chimeric strain of Staphylococcus aureus sequence type 80. PLoS One. 2020 Oct 14;15(10):e0232071. doi: 10.1371/journal.pone.0232071
    12. Roberts MC, Joshi PR, Monecke S, Ehricht R, Müller E, Gawlik D, Diezel C, Braun SD, Paudel S, Acharya M, Khanal L, Koju NP, Chalise M, Kyes RC. (2020) Staphylococcus aureus and Methicillin Resistant S. aureus in Nepalese Primates: Resistance to Antimicrobials, Virulence, and Genetic Lineages. Antibiotics (Basel). 2020 Oct 13;9(10):689. doi: 10.3390/antibiotics9100689
    13. Senok A, Nassar R, Celiloglu H, Nabi A, Alfaresi M, Weber S, Rizvi I, Müller E, Reissig A, Gawlik D, Monecke S, Ehricht R. (2020) Genotyping of methicillin resistant Staphylococcus aureus from the United Arab Emirates. Nature Sci Rep. 2020 Oct 29;10(1):18551. doi: 10.1038/s41598-020-75565-w
    14. Mcmanus BA, Aloba BK, Earls MR, Brennan GI, O'Connell B, Monecke S, Ehricht R, Shore AC, Coleman DC.(2020) Multiple distinct outbreaks of Panton-Valentine leukocidin (PVL)-positive community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in Ireland investigated by whole-genome sequencing. J Hosp Infect. 2020 Nov 28:S0195-6701(20)30545-4. doi: 10.1016/j.jhin.2020.11.021
    15. Monecke S, Syed MA, Khan MA, Ahmed S, Tabassum S, Gawlik D, Muller E, Reissig A, Braun SD, Ehricht R. Genotyping of methicillin-resistant Staphylococcus aureus from sepsis patients in Pakistan and detection of antibodies against staphylococcal virulence factors. Eur J Clin Microbiol Infect Dis. 2020;39(1):85-92. Epub 2019/09/05. doi: 10.1007/s10096-019-03695-9. PubMed PMID: 31482419.
    16. Sacco SC, Velazquez NS, Renna MS, Beccaria C, Baravalle C, Pereyra EAL, Monecke S, Calvinho LF, Dallard BE. Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages. Microb Pathog. 2020;142:104017. Epub 2020/02/02. doi: 10.1016/j.micpath.2020.104017. PubMed PMID: 32006636.
    17. Zurn K, Lander F, Hufnagel M, Monecke S, Berner R. Microarray Analysis of Group B Streptococci Causing Invasive Neonatal Early- and Late-onset Infection. Pediatr Infect Dis J. 2020;39(5):449-53. Epub 2020/02/25. doi: 10.1097/INF.0000000000002627. PubMed PMID: 32091495.
    18. Ahmed W, Neubauer H, Tomaso H, El Hofy FI, Monecke S, Abdeltawab AA, Hotzel H. Characterization of Staphylococci and Streptococci Isolated from Milk of Bovides with Mastitis in Egypt. Pathogens. 2020;9(5). Epub 2020/05/21. doi: 10.3390/pathogens9050381. PubMed PMID: 32429272; PubMed Central PMCID: PMCPMC7281669.
    19. Raafat D, Mrochen DM, Al'Sholui F, Heuser E, Ryll R, Pritchett-Corning KR, Jacob J, Walther B, Matuschka FR, Richter D, Westerhus U, Pikula J, van den Brandt J, Nicklas W, Monecke S, Strommenger B, van Alen S, Becker K, Ulrich RG, Holtfreter S. Molecular Epidemiology of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus in Wild, Captive and Laboratory Rats: Effect of Habitat on the Nasal S. aureus Population. Toxins (Basel). 2020;12(2). Epub 2020/01/30. doi: 10.3390/toxins12020080. PubMed PMID: 31991690; PubMed Central PMCID: PMCPMC7076793.
  • 2019
    1. Bai X, Zhang J, Ambikan A, Jernberg C, Ehricht R, Scheutz F, Xiong Y, Matussek A: Molecular Characterization and Comparative Genomics of Clinical Hybrid Shiga Toxin-Producing and Enterotoxigenic Escherichia coli (STEC/ETEC) Strains in Sweden. Sci Rep 2019, 9(1):5619.
    2. Earls MR, Shore AC, Brennan GI, Simbeck A, Schneider-Brachert W, Vremera T, Dorneanu OS, Slickers P, Ehricht R, Monecke S et al: A novel multidrug-resistant PVL-negative CC1-MRSA-IV clone emerging in Ireland and Germany likely originated in South-Eastern Europe. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 2019, 69:117-126.
    3. Khan FA, Hellmark B, Ehricht R, Soderquist B, Jass J: Related carbapenemase-producing Klebsiella isolates detected in both a hospital and associated aquatic environment in Sweden. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology 2018, 37(12):2241-2251.
    4. Krapf M, Muller E, Reissig A, Slickers P, Braun SD, Muller E, Ehricht R, Monecke S: Molecular characterisation of methicillin-resistant Staphylococcus pseudintermedius from dogs and the description of their SCCmec elements. Veterinary microbiology 2019, 233:196-203.
    5. Loncaric I, Kubber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Frotscher R, Harrison EM, Holmes MA et al: Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. Veterinary microbiology 2019, 230:138-144.
    6. Loreck K, Mitrenga S, Meemken D, Heinze R, Reissig A, Mueller E, Ehricht R, Engemann C, Greiner M: Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. PloS one 2019, 14(5):e0217290.
    7. Roberts MC, Fessler AT, Monecke S, Ehricht R, No D, Schwarz S: Molecular Analysis of Two Different MRSA Clones ST188 and ST3268 From Primates (Macaca spp.) in a United States Primate Center. Front Microbiol 2018, 9:2199.
    8. Weiss D, Gawlik D, Hotzel H, Engelmann I, Mueller E, Slickers P, Braun SD, Monecke S, Ehricht R: Fast, economic and simultaneous identification of clinically relevant Gram-negative species with multiplex real-time PCR. Future Microbiol 2019, 14:23-32.
    9. Pahlow S, Lehniger L, Hentschel S, Seise B, Braun SD, Ehricht R, Berg A, Popp J, Weber K: Rapid Isolation and Identification of Pneumonia-Associated Pathogens from Sputum Samples Combining an Innovative Sample Preparation Strategy and Array-Based Detection. ASC Omega 2019, 4(6):10362
    10. Coleman DC, Shore AC, Goering RV, Monecke S: Editorial: New Insights and Updates on the Molecular Epidemiology and Antimicrobial Resistance of MRSA in Humans in the Whole-Genome Sequencing Era. Front Microbiol 2019, 10:637.
    11. Dimitriou V, Biehl L, Hamprecht A, Vogel W, Dörfel D, Peter S, Schafhausen P, Rohde H, von Lilienfeld-Toal M, Klassert T et al: Controlling intestinal colonisation of high-risk haematology patients with ESBL-producing Enterobacteriaceae – a randomised, placebo controlled, multicentre, phase II trial (CLEAR). J Antimicrob Chemother 2019, dkz124, https://doi.org/10.1093/jac/dkz124External link.
    12. Hagel S, Makarewicz O, Hartung A, Weiss D, Stein C, Brandt C, Schumacher U, Ehricht R, Patchev V, Pletz MW: ESBL colonization and acquisition in a hospital population: The molecular epidemiology and transmission of resistance genes. PloS one 2019, 14(1):e0208505.
    13. Olofsson M, Matussek A, Ehricht R, Lindgren PE, Ostgren CJ: Differences in molecular epidemiology of Staphylococcus aureus and Escherichia coli in nursing home residents and people in unassisted living situations. J Hosp Infect 2019, 101(1):76-83.
    14. Thunberg U, Hugosson S, Fredlund H, Cao Y, Ehricht R, Monecke S, Mueller E, Engelmann S, Söderquist B: Anti-Staphylococcal humoral immune response in patients with chronic rhinosinusitis. Rhinology online 2019, 2:50-58.
    15. Tuchscherr L, Pollath C, Siegmund A, Deinhardt-Emmer S, Hoerr V, Svensson CM, Thilo Figge M, Monecke S, Loffler B: Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host. Toxins (Basel) 2019, 11(3).
    16. Weiss D, Gawlik D, Hotzel H, Engelmann I, Mueller E, Slickers P, Braun SD, Monecke S, Ehricht R: Fast, economic and simultaneous identification of clinically relevant Gram-negative species with multiplex real-time PCR. Future Microbiol 2019, 14:23-32.

44 Publikationen filtern

Die Publikationen filtern

  1. Methicillin-Resistant Staphylococcus aureus: The Shifting Landscape in the United Arab Emirates

    Year of publicationPublished in:Antibiotics S. Boucherabine, R. Nassar, L. Mohamed, M. Habous, A. Nabi, R. Husain, M. Alfaresi, S. Oommen, H. Khansaheb, M. Al Sharhan, H. Celiloglu, M. Raja, E. Abdelkarim, N. Ali, S. Tausif, V. Olowoyeye, N. Soares, M. Hachim, D. Moradigaravand, D. Everett, E. Mueller, S. Monecke, R. Ehricht, A. Senok
    Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant burden globally, particularly in the Arabian Gulf region. The United Arab Emirates (UAE) has experienced rising MRSA prevalence, with increasing diversity in the clonal complexes (CCs) identified. The COVID-19 pandemic, with its increased hospitalization rates and antibiotic use, may have further influenced MRSA’s genetic evolution and epidemiology in the country. Methods: To investigate this influence, genomic profiling of 310 MRSA clinical isolates collected between February and November 2022 was performed using a DNA microarray-based assay. Results: Isolates were assigned to 22 clonal complexes and 72 distinct strain assignments. The predominant clonal complexes were CC5, CC6, CC361, CC22, CC1, and CC8. Community-acquired MRSA lineages were dominant, with only one healthcare-associated MRSA lineage isolate identified. Upward trends of CC1153 were observed along with rare CCs, such as CC121-MRSA and CC7-MRSA, with the latter being reported for the first time in the Arabian Gulf region. The presence of pandemic strains USA300 CC8-MRSA-[IVa + ACME1] and CC8-MRSA-IV strains were also observed, including variants lacking Panton–Valentine leukocidin (pvl) genes and missing tst1 or enterotoxin genes. The PVL-negative CC772-MRSA-V/VT was identified, representing its first report in the UAE. A novel variant, CC361-MRSA-IV (tst1+/PVL+), was identified. Pvl genes were observed in 36% of the isolates, primarily from skin and soft tissue infections, while fusC (SCC-borne fusidic acid resistance) was identified in 13% of the isolates. Conclusions: The findings highlight the ongoing evolution of MRSA in the UAE, with the persistence and emergence of diverse and rare clonal complexes, driving the need for continuous genomic surveillance.
    University Bibliography Jena:
    fsu_mods_00019508External link

  2. Rapid Detection of Panton–Valentine Leukocidin Production in Clinical Isolates of Staphylococcus aureus from Saxony and Brandenburg and Their Molecular Characterisation †

    Year of publicationPublished in:Pathogens E. Müller, S. Monecke, M. Armengol Porta, M. Narvaez Encalada, A. Reissig, L. Rüttiger, P. Schröttner, I. Schwede, H. Söffing, A. Thürmer, R. Ehricht
    Panton–Valentine leukocidin (PVL) is a staphylococcal toxin associated with chronic/recurrent skin and soft tissue infections (SSTIs) and necrotizing pneumonia. Its detection in clinical isolates of Staphylococcus aureus warrants aggressive therapy and infection control measures. However, PVL detection relies on molecular methods of limited use, especially in outpatient or resource-poor settings. In order to aid the development of a lateral flow (LF) test for PVL, clinical isolates from SSTIs were collected in 2020/21 at three laboratories in two cities in the Eastern part of Germany. After the exclusion of duplicate and serial isolates, 83 isolates were eligible. These were tested using an experimental LF test for PVL production. They were also characterized using DNA microarrays, facilitating the detection of virulence and resistance markers as well as the assignment to clonal complexes and epidemic/pandemic strains. Thirty-nine isolates (47%) were PVL-positive, and the LF results were in 81 cases (97.6%) concordant with genotyping. One false-positive and one false-negative case were observed. This translated into a diagnostic sensitivity of 0.974 and a diagnostic specificity of 0.977. The most common PVL-positive MSSA lineages were CC152 (n = 6), CC121 (n = 4), and CC5 and CC30 (each n = 2). Thirty isolates (36%) were mecA-positive. The MRSA rate among PVL-negatives was 20% (nine isolates), but among the PVL-positives, it was as high as 54% (n = 21). The most common PVL-MRSA strains were CC398-MRSA-VT (n = 5), CC5-MRSA-IV “Sri Lanka Clone” (n = 4), CC8-MRSA-[mec IV+Hg] “Latin American USA300” (n = 4), and CC22-MRSA-IV (PVL+/tst+) (n = 2). While the PVL rate was similar just like the German isolates from a previous study a decade before, the MRSA rate among PVL-positives was clearly higher. All PVL-MRSA strains detected, as well as the most common methicillin-susceptible lineage (CC152), are known to be common locally in other parts of the world, and might, thus, be regarded as travel-associated. Therefore, patients with suspected PVL-associated disease should be asked for their history of travel or migration, and, in case of hospitalization, they should be treated as MRSA cases until proven otherwise.
    University Bibliography Jena:
    fsu_mods_00023574External link

  3. Rapid Simultaneous Detection of the Clinically Relevant Carbapenemase Resistance Genes blaKPC, blaOXA48, blaVIM and blaNDM with the Newly Developed Ready-to-Use qPCR CarbaScan LyoBead

    Year of publicationPublished in:International Journal of Molecular Sciences M. Reinicke, C. Diezel, S. Teimoori, B. Haase, S. Monecke, R. Ehricht, S. Braun
    Antibiotic resistance, in particular the dissemination of carbapenemase-producing organisms, poses a significant threat to global healthcare. This study introduces the qPCR CarbaScan LyoBead assay, a robust, accurate, and efficient tool for detecting key carbapenemase genes, including blaKPC, blaNDM, blaOXA-48, and blaVIM. The assay utilizes lyophilized beads, a technological advancement that enhances stability, simplifies handling, and eliminates the need for refrigeration. This feature renders it particularly well-suited for point-of-care diagnostics and resource-limited settings. The assay’s capacity to detect carbapenemase genes directly from bacterial colonies without the need for extensive sample preparation has been demonstrated to streamline workflows and enable rapid diagnostic results. The assay demonstrated 100% specificity and sensitivity across a diverse range of bacterial strains, including multiple allelic variants of target genes, facilitating precise identification of resistance mechanisms. Bacterial strains of the species Acinetobacter baumannii, Citrobacter freundii, Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae and Pseudomonas aeruginosa were utilized as reference material for assay development (n = 9) and validation (n = 28). It is notable that the assay’s long shelf life and minimal operational complexity further enhance its utility for large-scale implementation in healthcare, food safety, and environmental monitoring. The findings emphasize the necessity of continuous surveillance and the implementation of rapid diagnostic methods for the effective detection of resistance genes. Furthermore, the assay’s potential applications in other fields, such as toxin-antitoxin system research and monitoring of resistant bacteria in the community, highlight its versatility. In conclusion, the qPCR CarbaScan LyoBead assay is a valuable tool that can contribute to the urgent need to combat antibiotic resistance and improve global public health outcomes.
    University Bibliography Jena:
    fsu_mods_00020890External link

  4. Multiplex Real-Time Polymerase Chain Reaction and Recombinase Polymerase Amplification: Methods for Quick and Cost-Effective Detection of Vancomycin-Resistant Enterococci (VRE)

    Year of publicationPublished in:Antibiotics I. Osadare, A. Abdilahi, M. Reinicke, C. Diezel, M. Collatz, A. Reissig, S. Monecke, R. Ehricht
    Background/Objectives: Vancomycin-resistant enterococci (VRE) are one of the leading causes of antibiotic-resistant infections in the hospital setting worldwide, and this has become a major issue, because most patients infected with this strain are difficult to treat. Multiplex real-time polymerase chain reaction (RT PCR) is an advantageous technique that can amplify multiple targets in a single reaction, and can be used to quickly detect specific targets in VRE within two hours, starting from suspected colonies of bacterial cultures, without sample preparation. Methods: In this study, we selected the glycopeptide/vancomycin resistance genes that are most common in clinical settings, vanA and vanB, in combination with the species markers ddl_faecium and ddl_faecalis for the most common VRE species—Enterococcus faecium and Enterococcus faecalis. Results: DNA from forty clinical VRE strains was prepared using a fast and economic heat lysis method, and a multiplex real-time PCR assay was optimized and carried out subsequently. The results were in concordance with the results from recombinase polymerase amplification (RPA) of the same VRE samples. Conclusions: Multiplex RT PCR and RPA for VRE detection proffers a second method for the confirmation of vancomycin resistance, and it can be developed as a fast screening assay for patients before admission into high-risk settings.
    University Bibliography Jena:
    fsu_mods_00023470External link

  5. Characterisation of Staphylococcus aureus Strains and Their Prophages That Carry Horse-Specific Leukocidin Genes lukP/Q

    Year of publicationPublished in:Toxins S. Monecke, S. Burgold-Voigt, A. Feßler, M. Krapf, I. Loncaric, E. Liebler-Tenorio, S. Braun, C. Diezel, E. Müller, M. Reinicke, A. Reissig, A. Cabal Rosel, W. Ruppitsch, H. Hotzel, D. Hanke, C. Cuny, W. Witte, S. Schwarz, R. Ehricht
    Leukocidins of Staphylococcus (S.) aureus are bicomponent toxins that form polymeric pores in host leukocyte membranes, leading to cell death and/or triggering apoptosis. Some of these toxin genes are located on prophages and are associated with specific hosts. The genes lukP/Q have been described from equine S. aureus isolates. We examined the genomes, including the lukP/Q prophages, of S. aureus strains belonging to clonal complexes CC1, CC350, CC816, and CC8115. In addition to sequencing, phages were characterised by mitomycin C induction and transmission electron microscopy (TEM). All lukP/Q prophages integrated into the lip2=geh gene, and all included also the gene scn-eq encoding an equine staphylococcal complement inhibitor. The lukP/Q prophages clustered, based on gene content and allelic variants, into three groups. One was found in CC1 and CC97 sequences; one was present mainly in CC350 but also in other lineages (CC1, CC97, CC133, CC398); and a third one was exclusively observed in CC816 and CC8115. Prophages of the latter group additionally included a rare enterotoxin A allele (sea₃₂₀E). Moreover, a prophage from a CC522 goat isolate was found to harbour lukP. Its lukF component could be regarded as chimaera comprising parts of lukQ and of lukF-P83. A putative kinase gene of 1095 basepairs was found to be associated with equine strains of S. aureus. It was also localised on prophages. However, these prophages were different from the ones that carried lukP/Q, and three different integration sites of kinase-carrying phages were identified. These observations confirmed the presence of prophage-located important virulence-associated genes in equine S. aureus and that certain prophages might determine the host specificity of the staphylococcal strains they reside in.
    University Bibliography Jena:
    fsu_mods_00019634External link

  6. Staphylococcus aureus in Rwandan dogs predominantly representing human-associated lineages

    Year of publicationStatusReview pendingPublished in:Letters in applied microbiology / Society for Applied Microbiology, sfam L. Marek, E. Irimaso, J. Turikumwenayo, B. Mukamulisa, P. Ndishimye, F. Muragijemariya, A. Cabal-Rosel, A. Desvars-Larrive, O. Fischer, M. Szostak, E. Müller, S. Braun, M. Ehling-Schulz, J. Spergser, T. Grunert, W. Ruppitsch, A. Feßler, S. Schwarz, S. Monecke, R. Ehricht, F. Künzel, I. Loncaric
    The present study aimed at examining the nasal and ear carriage of Staphylococcus aureus of Rwandan dogs and cats. Sixty-five S. aureus isolates were detected, all originating from the nostrils of dogs. Resistance to penicillin, penicillin/erythromycin/clindamycin, penicillin/tetracycline, and tetracycline solely was observed. The isolates were assigned to 23 different spa types, among them three novel (t21589, t21661, and t21662) variants, associated with eleven clonal complexes (CCs) (CC1, CC5, CC12, CC15, CC22, CC30, CC45, CC97, CC152, CC707, and CC834). Four isolates could not be assigned to any known CC. MLST revealed that one of them belonged to the new sequence type (ST) 9069. Panton-Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83), the toxic shock syndrome toxin gene tst-1, and various virulence-associated genes were detected. These findings demonstrate the dogs are colonized with human lineages of S. aureus. Coupled with the limited availability of S. aureus data from human medicine in Rwanda underscores the importance of hygiene measures and supports the need for a rigorous One-Health Surveillance program of the companion animals-human interface.
    University Bibliography Jena:
    fsu_mods_00024917External link

  7. Diversity of Staphylococcus aureus isolated from nares of ruminants

    Year of publicationPublished in:Journal of applied microbiology I. Loncaric, H. Keinprecht, E. Irimaso, A. Cabal-Rosel, B. Stessl, C. Ntakirutimana, L. Marek, O. Fischer, M. Szostak, C. Oberrauch, T. Wittek, E. Müller, A. Desvars-Larrive, A. Feßler, S. Braun, S. Schwarz, M. Ehling-Schulz, S. Monecke, R. Ehricht, W. Ruppitsch, T. Grunert, J. Spergser
    University Bibliography Jena:
    fsu_mods_00009849External link

  8. From Shadows to Spotlight: Enhancing Bacterial DNA Detection in Blood Samples through Cutting-Edge Molecular Pre-Amplification

    Year of publicationPublished in:Antibiotics M. Reinicke, S. Braun, C. Diezel, O. Lemuth, I. Engelmann, T. Liebe, R. Ehricht
    One of the greatest challenges to the use of molecular methods for diagnostic purposes is the detection of target DNA that is present only in low concentrations. One major factor that negatively impacts accuracy, diagnostic sensitivity, and specificity is the sample matrix, which hinders the attainment of the required detection limit due to the presence of residual background DNA. To address this issue, various methods have been developed to enhance sensitivity through targeted pre-amplification of marker sequences. Diagnostic sensitivity to the single molecular level is critical, particularly when identifying bloodstream infections. In cases of clinically manifest sepsis, the concentration of bacteria in the blood may reach as low as one bacterial cell/CFU per mL of blood. Therefore, it is crucial to achieve the highest level of sensitivity for accurate detection. In the present study, we have established a method that fills the analytical gap between low concentrations of molecular markers and the minimum requirements for molecular testing. For this purpose, a sample preparation of whole blood samples with a directly downstream pre-amplification was developed, which amplifies specific species and resistance markers in a multiplex procedure. When applying pre-amplification techniques, the sensitivity of the pathogen detection in whole blood samples was up to 100 times higher than in non-pre-amplified samples. The method was tested with blood samples that were spiked with several Gram-positive and Gram-negative bacterial pathogens. By applying this method to artificial spiked blood samples, it was possible to demonstrate a sensitivity of 1 colony-forming unit (CFU) per millilitre of blood for S. aureus and E. faecium. A detection limit of 28 and 383 CFU per ml of blood was achieved for E. coli and K. pneumoniae, respectively. If the sensitivity is also confirmed for real clinical blood samples from septic patients, the novel technique can be used for pathogen detection without cultivation, which might help to accelerate diagnostics and, thus, to decrease sepsis mortality rates.
    University Bibliography Jena:
    fsu_mods_00011509External link

  9. A Proof-of-Concept Protein Microarray-Based Approach for Serotyping of Salmonella enterica Strains

    Year of publicationPublished in:Pathogens S. Braun, E. Müller, K. Frankenfeld, D. Gary, S. Monecke, R. Ehricht
    Salmonella enterica, a bacterium causing foodborne illnesses like salmonellosis, is prevalent in Europe and globally. It is found in food, water, and soil, leading to symptoms like diarrhea and fever. Annually, it results in about 95 million cases worldwide, with increasing antibiotic resistance posing a public health challenge. Therefore, it is necessary to detect and serotype Salmonella for several reasons. The identification of the serovars of Salmonella enterica isolates is crucial to detect and trace outbreaks and to implement effective control measures. Our work presents a protein-based microarray for the rapid and accurate determination of Salmonella serovars. The microarray carries a set of antibodies that can detect different Salmonella O- and H-antigens, allowing for the identification of multiple serovars, including Typhimurium and Enteritidis, in a single miniaturized assay. The system is fast, economical, accurate, and requires only small sample volumes. Also, it is not required to maintain an extensive collection of sera for the serotyping of Salmonella enterica serovars and can be easily expanded and adapted to new serovars and sera. The scientific state of the art in Salmonella serotyping involves the comparison of traditional, molecular, and in silico methods, with a focus on economy, multiplexing, accuracy, rapidity, and adaptability to new serovars and sera. The development of protein-based microarrays, such as the one presented in our work, contributes to the ongoing advancements in this field.
    University Bibliography Jena:
    fsu_mods_00013340External link

  10. Survey in ruminants from Rwanda revealed high diversity and prevalence of extended-spectrum cephalosporin-resistant Enterobacterales

    Year of publicationPublished in:BMC Veterinary Research E. Irimaso, H. Keinprecht, M. Szostak, A. Rosel, B. Stessl, A. Desvars-Larrive, C. Ntakirutimana, O. Fischer, T. Wittek, E. Müller, A. Feßler, S. Braun, S. Schwarz, S. Monecke, R. Ehricht, J. Spergser, W. Ruppitsch, I. Loncaric
    Background: Antimicrobial resistance (AMR) in Enterobacterales constitutes a significant threat to the health of both humans and animals and a socioeconomic problem. Enterobacterales, mainly Escherichia coli, carrying β-lactamases has become one of the main indicators to estimate the burden of AMR in animals within “One Health” approach. Objectives: To assess the presence of extended-spectrum cephalosporin-resistant Enterobacterales associated with ruminants (cattle, sheep, goats) habituated in all five provinces of Rwanda and to perform in depth characterization of isolates. Methods: We screened 454 rectal swabs from 203 cows, 170 goats, and 81 sheep and selective isolation of extended-spectrum cephalosporin-resistant Enterobacterales was conducted. Isolates were identified as a members of the order Enterobacterales by MALDI-TOF MS and further characterized by susceptibility testing and by whole-genome sequencing. Results: Out of the 454 samples, 64 extended-spectrum cephalosporin-resistant Enterobacterales were isolated from 58 animals. Isolates belonged to seven bacterial species and were identified as Escherichia coli (n = 54), Enterobacter bugandensis (n = 4), Enterobacter mori (n = 2), Klebsiella pneumoniae (n = 2), Enterobacter dykesii (n = 1), and Citrobacter freundii (n = 1). All isolates displayed an Extended-spectrum β-lactamases (ESBL) phenotype, with exception of Citrobacter freundii isolate displayed both an ESBL and AmpC phenotype. In addition, all Enterobacter isolates were identified as stably de-repressed AmpC-producers. ESBLs genes, blaCTX−M−₁₅ was predominant. Resistance to tetracycline and tet(A) was most frequently observed among non-β-lactam resistance. Forty-eight isolates displayed multidrug-resistance phenotypes. A shiga toxin-producing E. coli and an enterotoxigenic E. coli isolate were observed. Genome comparisons revealed thirty-five E. coli sequence types (ST) (ST10, ST307 being predominate). Conclusions: Considering the high proximity between ruminants and humans in Rwanda, the dissemination of antimicrobial drug resistance highlights the public health threats and requires the joint and multisectoral action of human and veterinary medicine, at human-animal-environment interfaces. Therefore, it is important to establish national and global “One Health” surveillance programs of AMR to tackle the antibiotic-resistant crisis in human and veterinary medicine.
    University Bibliography Jena:
    fsu_mods_00018379External link

  11. Characterizing Methicillin-Resistant Staphylococcus spp. and Extended-Spectrum Cephalosporin-Resistant Escherichia coli in Cattle

    Year of publicationPublished in:Animals L. Abdank, I. Loncaric, S. Braun, E. Müller, S. Monecke, R. Ehricht, R. Krametter-Frötscher
    In the field of cattle medicine in Austria, to date, few studies have investigated the presence of methicillin-resistant Staphylococcus aureus and extended-spectrum β-lactamase-producing Escherichia coli in Austria. For this reason, milk and nasal samples were examined for the presence of methicillin-resistant Staphylococcus aureus as well as fecal samples for extended-spectrum cephalosporin-resistant Escherichia coli. The nasal and fecal swabs were collected during the veterinary treatment of calf pneumonia and calf diarrhea. For the milk samples, the first milk jets were milked into a pre-milking cup and then the teats were cleaned and disinfected before the samples were taken. The cows were selected during the veterinary visits to the farms when treatment was necessary due to mastitis. Depending on the severity of the mastitis (acute mastitis or subclinical mastitis), antibiotics and non-steroidal anti-inflammatory drugs were given immediately (acute disease) or after completion of the antibiogram (subclinical disease). Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. No methicillin-resistant Staphylococcus aureus was found in the milk samples, but one nasal swab was positive for methicillin-resistant Staphylococcus aureus. Twenty-two Escherichia coli isolates were detected among the fecal samples. All the Escherichia coli isolates were resistant to ceftazidime. In all the Escherichia coli isolates, genes from the blaCTX family were detected with other bla genes or alone; the most frequently observed β-lactamase gene was blaCTX₋M₋₁/₁₅ (n = 20). In total, 63.6% (n = 14) of the isolates exhibited a multidrug-resistant phenotype and one E. coli isolate (4.5%) harbored the AmpC gene. Precisely because the presence of data regarding extended-spectrum cephalosporin-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus in calves and cows in Austria is rare, this study further expands our understanding of antimicrobial resistance in Austrian cattle, which is highly relevant for successful antibiotic therapy in sick cattle.
    University Bibliography Jena:
    fsu_mods_00018893External link

  12. Diversity of Staphylococcus aureus associated with mastitis from dairy cows in Rwanda

    Year of publicationPublished in:Journal of Global Antimicrobial Resistance H. Keinprecht, E. Irimaso, A. Rosel, B. Stessl, C. Ntakirutimana, L. Marek, O. Fischer, M. Szostak, J. Zöchbauer, T. Wittek, E. Müller, A. Desvars-Larrive, A. Feßler, S. Braun, S. Schwarz, J. Spergser, M. Ehling-Schulz, S. Monecke, R. Ehricht, W. Ruppitsch, T. Grunert, I. Loncaric
    Objectives: The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda. Methods: A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping). Results: Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton–Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97. Conclusion: The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine–human interface.
    University Bibliography Jena:
    fsu_mods_00011562External link
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